|Table of Contents|

The effects of G-CSF on the regulation of CXCR-4 expression and chemotaxis in leukemia cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2016 06
Page:
858-861
Research Field:
Publishing date:

Info

Title:
The effects of G-CSF on the regulation of CXCR-4 expression and chemotaxis in leukemia cells
Author(s):
Dai Zhiming; Niu Ben; Zhang Wanggang; Liu Jie; Wang Jin
Department of Hematology，2nd Hospital Affiliated of Xi'an Jiaotong University，Shaanxi Xi'an 710004,China．
Keywords:
acute leukemia; CXCR4; SDF-1; G-CSF; immune micro-environmental
PACS:
R733.71
DOI:
10.3969/j.issn.1672-4992.2016.06.003
Abstract:
Objective:To investigate the effects of granulocyte colony-stimulating factor (G-CSF) on the regulation of C-X-C chemokine receptor type 4 (CXCR4) expression and chemotaxis in U937 cell and WEHI-3 cell line.Methods:Co-culture U937 cell and WEHI-3 cell with G-CSF for 6 hours,12 hours,18 hours,24 hours.Flow cytometer (FCM) was used to detect the expression of CXCR4，and microporous cell transfer experiment to observe migration ability of U937 cell and WEHI-3 cell.Results:G-CSF inhibited the expression of CXCR4 in U937 cell and WEHI-3 cell with extension of time co-culture.After cultured 18 hours with G-CSF,the expression level of CXCR4 decreased to a minimum (P＜0.05).The cells entered the lower chamber of Transwell were significantly decreased between co-culture 18 hours with G-CSF and without G-CSF (P＜0.05).Instead,the cell without co-cultur with G-CSF had stronger ability to migrate to the stromal cell-derived factor-1 (SDF-1) than that cell cultured with G-CSF (P＜0.05).Conclusion:G-CSF not only down-regulated expression of cell surface CXCR4 but also inhibited the cell migration to SDF-1 in U937 cell and WEHI-3 cell.G-CSF might suppress the immune escape of leukemia cells by regulating the immune micro-environmental of bone marrow,which may provide a new strategy for treatment of leukemia.

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Last Update: 2016-01-29