|Table of Contents|

The impact on the expression of apoptosis related genes after MDA-MB-231 regulated by Ghrelin of proliferated concentration

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2016 06
Page:
854-857
Research Field:
Publishing date:

Info

Title:
The impact on the expression of apoptosis related genes after MDA-MB-231 regulated by Ghrelin of proliferated concentration
Author(s):
Yang Lei; Tu Wei; Jin Guanghua; Wen Jian; Fan Ying; Qu Wenzhi
Department of Breast Surgery,The Fourth Affiliated Hospital of China Medical University,Liaoning Shenyang 110032,China.
Keywords:
Ghrelin; MDA-MB-231 cells; Bcl-2; Bax
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2016.06.002
Abstract:
Objective:To explore the effect on the gene expression related to apoptosis,such as Bcl-2 and Bax,when MDA-MB-231 cells are regulated by Ghrelin of proliferated concentration.Methods:We observed the n-octanoylated Ghrelin affected MDA-MB-231 cells by cell culture,MTT and flow cytometry was used to test the alteration of related apoptosis proterin.Results:When the concentration of Ghrelin ranged from 0nmol/L~100nmol/L,Ghrelin promoted MDA-MB-231 cells proliferation,however when its concentration was between 100nmol/L and 1 000nmol/L,Ghrelin inhibited cells proliferation,and we also found that Ghrelin had the strongest action of proliferation-promoting at 100nmol/L.The experimental group (100nmol/L Ghrelin deal with MDA-MB-231 cells) campared with control group (MDA-MB-231 cells without Ghrelin) had significant statistics difference,the ratio of Bcl-2/Bax in experimental group was higher than control group,with significant difference.Conclusion:Lower Ghrelin concentration can promote MDA-MB-231 cells proliferation with rised Bcl-2/Bax ratio,we suppose that Ghrelin promote MDA-MB-231 cells proliferation have relationship with the release of mitochondria antiapoptotic factors.

References:

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Last Update: 2016-01-29