|Table of Contents|

The expression and function of microRNA-130a in non-small cell lung cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 13
Page:
1838-1841
Research Field:
Publishing date:

Info

Title:
The expression and function of microRNA-130a in non-small cell lung cancer
Author(s):
Zhang Haitao; Wang Cuicui; Ren Lingyan; Gui Weiwei; Feng Hui
Department of Oncology，Chinese People's Liberation Army 454th Hospital，Jiangsu Nanjing 210002，China.
Keywords:
NSCLC; microRNA-130a; Smad4; proliferation
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2015.13.16
Abstract:
Objective：To investigate the expression of miR-130a in patients with non-small cell lung cancer （NSCLC) and to analyze the function and mechanism of miR-130a.Methods：The expression levels of miR-130a in 50 tumor tissues and paired normal tissues of NSCLC were detected by using RT-PCR.The relationship between the level of miR-130a and the pathological characteristics was analyzed.miR-130a in vitro was transiently transferred into A549 cells．The expression level of miR-130a in A549 cells was detected by RT-PCR．Cell viability was analyzed by CCK8 assay．Expression of Smad4 protein was detected by Western blot．Results：The expression level of miR-130a was up-regulated in tumor tissues as compared with normal lung tissues （P<0.05).The expression of miR-130a was associated with tumor size，lymphatic metastasis，and TNM stages （P<0.05).miR-130a in anti-miR-130a-transfected A549 cells was significantly decreased compared with the control group (P<0.05)．The proliferation of anti-miR-130a-transfected A549 cells was inhibited significantly.The expression level of Smad4 was markedly up-regulated after transfection （P<0.05)．Conclusion：The expression of miR-130a was up-regulated in NSCLC，miR-130a might promote proliferation and metastasis of lung cancer in part by down-regulating the expression of Smad4.

References:

［1］Tanoue LT.Staging of non-small cell lung cancer［J］.Semin Respir Crit Care Med，2008，29(3)：248-260.
［2］Jeong HC.Clinical spect of microRNA in lung cancer［J］.Tuberc Respir Dis（Seoul)，2014，77(2)：60-64.
［3］Larsen MT，Hger M，Glenthj A，et al.miRNA-130a regulates C/EBP-ε expression during granulopoiesis［J］.Blood，2014，123(7)：1079-1089.
［4］Li B，Huang P，Qiu J，et al.MicroRNA-130a is down-regulated in hepatocellular carcinoma and associates with poor prognosis［J］.Med Oncol，2014，31(10)：230.
［5］He L，Wang HY，Zhang L,et al.Prognostic significance of low DICER expression regulated by miR-130a in cervical cancer［J］.Cell Death Dis，2014，5：e1205.
［6］Zhang X，Huang L，Zhao Y，et al.Downregulation of miR-130a contributes to cisplatin resistance in ovarian cancer cells by targeting X-linked inhibitor of apoptosis （XIAP) directly［J］.Acta Biochim Biophys Sin，2013，45(12)：995-1001.
［7］Liu L，Nie J，Chen L，et al.The oncogenic role of microRNA-130a/301a/454 in human colorectal cancer via targeting Smad4 expression［J］.PLoS One,2013，8(2)：e55532.
［8］Qiu S，Lin S，Hu D，et al.Interactions of miR-323/miR-326/miR-329 and miR-130a/miR-155/miR-210 as prognostic indicators for clinical outcome of glioblastoma patients［J］.J Transl Med，2013，11：10.
［9］Boll K，Reiche K，Kasack K，et al.MiR-130a，miR-203 and miR-205 jointly repress keyoncogenic pathways and are downregulated in prostate carcinoma［J］.Oncogene，2013，32(3)：277-285.
［10］Kovaleva V，Mora R，Park YJ,et al.miRNA-130a targets ATG2B and DICER1 to inhibit autophagy and trigger killing of chronic lymphocytic.leukemia cells［J］.Cancer Res，2012，72(7)：1763-1772.
［11］Wang Sinian,Jiang Qisheng.Advance of the research on miRNA-130a and invasion in non-small cell lung cancinoma［J］.Mod Oncol,2013,21(4):903-905.［王思念,江其生.miRNA-130α与非小细胞肺癌侵袭转移相关研究进展［J］.现代肿瘤医学,2013,21(4):903-905.］
［12］Ke Z，Zhang X，Ma L，et al.Expression of DPC4/Smad4 in non-small-cell lung carcinoma and its relationship with angiogenesis［J］.Neoplasma，2008，55(4)：323-329.
［13］Yan Jidong，Zhang Zhiyong，Ren Shuhua，et al.The expression and significance of Smad4 mRNA in non-small cell lung cance［J］.China Tumor Clin,2011,38(12):700-703.［闫继东，张志勇，任淑华，等.Smad4 mRNA 在非小细胞肺癌中的表达及意义［J］.中国肿瘤临床,2011,38(12)：700-703.］

Memo

Memo:
-
Last Update: 2015-05-29