|Table of Contents|

Combination of Arsenic Trioxide and Celecoxib:Anti-tumor activity on epithelial ovarian cancer cell line and the mechanisms

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 13
Page:
1805-1809
Research Field:
Publishing date:

Info

Title:
Combination of Arsenic Trioxide and Celecoxib:Anti-tumor activity on epithelial ovarian cancer cell line and the mechanisms
Author(s):
Gao Kefei1Zeng Gen2
1.Department of Gynecology,The First Affiliated Hospital,Guangzhou University of Traditional Chinese Medicine,Guangdong Guangzhou 510405,China;2.Department of Gynecology and Obstetrics,Hainan Provincial Traditional Chinese Medicine Hospital,Hainan Haikou 570203,China.
Keywords:
Arsenic TrioxideCelecoxibovarian neoplasms
PACS:
R73-34;R737.31
DOI:
10.3969/j.issn.1672-4992.2015.13.07
Abstract:
Objective:To investigate the effects of the combination of Arsenic Trioxide and COX-2 inhibitor Celecoxib in epithelial ovarian cancer cells and the mechanisms.Methods:In OVCAR-3 cells,the dose-dependent anti-cancer effects were observed when AS2O3 or Celecoxib was applied as a single agent,and the combination effects of the two agents.Apoptosis and cell cycle status was tested by Flow Cytometry and Western blot were applied.Results:Dose-dependent anti-proliferative effects on OVCAR-3 cells were observed when AS2O3 or Celecoxib was applied as single agent,the IC50 value was 4.72μmol/L and 42.58μmol/L respectively.When the two agents were applied simultaneously,the growth inhibition rate was higher significantly than that of the group treated with single-agent AS2O3 or Celecoxib (P<0.05).The results of Flow Cytometry testing showed significant G2/M phase block and increased apoptosis rate when the two agents were applied simutaneously.COX-2 and bcl-2 expression of the two-agent treatment group was down-regulated,bax and caspase-3 were up-regulated compared with single-agent AS2O3 or Celecoxib treated group.Conclusion:There are synergistic effects when arsenic trioxide and Celecoxib were used in combination in epithelial ovarian cancer cell line.

References:

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Last Update: 2015-05-29