|Table of Contents|

The effect of silencing Dicer by RNA interference on proliferation and migration of human breast cancer cell

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 06
Page:
761-764
Research Field:
Publishing date:

Info

Title:
The effect of silencing Dicer by RNA interference on proliferation and migration of human breast cancer cell
Author(s):
Zhao Jianping
Department of Biotherapy,Central Hospital of Handan City,Hebei Handan 056001,China.
Keywords:
Dicer; proliferation; migration; breast cancer
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2015.06.10
Abstract:
Objective:To investigate the effect of silencing Dicer by RNAi on the biological characteristics of human breast cancer MCF-7 in vitro．Methods:Liposome mediated method was used to transfect siRNA sequence targeting Dicer as control into MCF-7 cells．Dicer expression levels after transfection were detected by using Real-time PCR and Western blot.The biological behavior cells of MCF-7 cells were evaluated by MTT and transwell assay．Results:Compared to control group,the mRNA levels of Dicer in siRNA group were significantly reduced at 24，48 and 72h after transfection(0.00152，0.00063，0.00096,respectively,P＜0.01),with the effect most obvious at 48h after transfection.The protein levels in siRNA group were also significantly reduced(2.581±0.028，P＜0.01).MTT assay revealed the absorbance value in siRNA group was significantly increased（P＜0.01),and Transwell assay showed the number of migratory cells in siRNA group was significantly increased at 48h after transfection.Conclusion:Silencing of Dicer significantly enhanced proliferation and migration of MFC-7 cells．

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河北省卫生厅科研基金项目支持课题（编号：ZD2014118）
Last Update: 2015-02-02