|Table of Contents|

The re-expression of FHIT raise the apoptosis of colon cancer SW480 cells reacted by Mitomycin C

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 06
Page:
759-761
Research Field:
Publishing date:

Info

Title:
The re-expression of FHIT raise the apoptosis of colon cancer SW480 cells reacted by Mitomycin C
Author(s):
Chen Jian1; Xue Yajing2; Sun Yuanyuan1; Shao Jianguo1
1.Department of Gastroenterology，Nantong Third People's Hospital，Jiangsu Nantong 226006，China；2.Clinical Laboratory of Nantong Tumor Hospital，Jiangsu Nantong 226000，China.
Keywords:
colonic neoplasm; genes; FHIT; transfection; Mitomycin C; apoptosis
PACS:
R735.35
DOI:
10.3969/j.issn.1672-4992.2015.06.09
Abstract:
Objective:To study the re-expression of FHIT raise the apoptosis of colon cancer SW480 cells reacted by Mitomycin C.Methods：Eukaryotic vector expressing FHIT gene and empty vector were transfected into human colon cancer SW480 cells.Then,the FHIT proteins were detected by Western blotting．Cell growth inhibition reacted by Mitomycin C was determined by MTT.Cell apoptosis was determined by flow cytometry analysis.Results：Marked expression of FHIT protein was detected in SW480 cells after transfection with PRC/CMV vector,but it was not observed in SW480 cells after transfection with empty vector．Cell growth inhibition of the FHIT+ SW480 cell treated with Mitomycin C was more obvious than FHIT- SW480 cell.The apoptosis of the FHIT+ SW480 cell treated with Mitomycin C was more obvious than FHIT- SW480 cell.Conclusion：FHIT gene transfected by plasmid don't increase the apoptosis of cell,but,FHIT gene transfection can increase drug sensitivity of MMC.

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Last Update: 2015-02-02