|Table of Contents|

Inhibitory effect of TSLC1 gene on proliferation and invasion ability in esophageal carcinoma cell EC109

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 06
Page:
755-759
Research Field:
Publishing date:

Info

Title:
Inhibitory effect of TSLC1 gene on proliferation and invasion ability in esophageal carcinoma cell EC109
Author(s):
Ran Yonggang1; You Yanjie2; Zhang Xiaohui3; Wang Haibo4; Guo Xu1; Zhao Xinlei1
1.Department of Teaching and Training，Bethune Military Medical NCO of PLA,Hebei Shijiazhuang 050081,China；2.Department of Pharmacy,Luohe Medical College,Henan Luohe 462000,China；3.Department of Radiation Oncology,Hospital Affiliated Henan Science Technology University,Henan Zhengzhou 471000,China；4.Department of Physiology，Bethune Military Medical NCO of PLA,Hebei Shijiazhuang 050081,China.
Keywords:
esophageal carcinoma; TSLC1; proliferation; invasion
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2015.06.08
Abstract:
Objective:To investigate the inhibitory effects of TSLC1 gene on proliferation and invasion of esophageal carcinoma cell line EC109.Methods:Coding area of TSLC1 gene was obtained by RT-PCR and cloned into eukaryotic expression vector pcDNA3.1(pcDNA3.1-TSLC1).Tumor cell activities of proliferation,adherence,invasion and migration were measured by MTT assay,adhesion assay,invasion or migration assay.Results：The eukaryotic expression vector was constructed correctly and stable transfection of pcDNA3.1-TSLC1 significantly suppressed the abilities of proliferation,adhesion,invasion and migration in EC109 cells(P＜0.05).Conclusion:Expression of TSLC1 gene has a significant inhibitory effect on the malignant phenotypes in nasopharyngeal carcinoma cell line EC109,provide an experimental basis for investigating effects of TSLC1 in the occurrence and development of esophageal carcinoma.

References:

［1］ Liu D,Feng X,Wu X,et al.Tumor suppressor in lung cancer 1 (TSLC1),a novel tumor suppressor gene,is implicated in the regulation of proliferation,invasion,cell cycle,apoptosis,and tumorigenicity in cutaneous squamous cell carcinoma［J］.Tumour Biol,2013,34(6):3773-3783.
［2］ Liang QL,Wang BR,Li ZY,et al.Effect of TSLC1 gene on growth and apoptosis in human esophageal carcinoma Eca109 cells［J］.Arch Med Sci,2012,8(6):987-992.
［3］ Lu B,Di W,Wang H,et al.Tumor suppressor TSLC1 is implicated in cell proliferation,invasion and apoptosis in laryngeal squamous cell carcinoma by regulating Akt signaling pathway［J］.Tumour Biol,2012,33(6):2007-2017.
［4］ Zhang J,Ning J,Geng J,et al.Down-regulation of tumor suppressor in lung cancer 1(TSLC1) expression correlates with poor prognosis in patients with colon cancer［J］.J Mol Histol,2012,43(6):715-721.
［5］ Yang G,He W,Cai M,et al.Loss/down-regulation of tumor suppressor in lung cancer 1 expression is associated with tumor progression and is a biomarker of poor prognosis in ovarian carcinoma［J］.Int J Gynecol Cancer,2011,21(3):486-493.
［6］ Liang QL,Chen GQ,Li ZY,et al.Function and histopathology of a cell adhesion molecule TSLC1 in cancer［J］.Cancer Invest,2011,29(2):107-112.
［7］ Chen K,Wang G,Peng L,et al.CADM1/TSLC1 inactivation by promoter hypermethylation is a frequent event in colorectal carcinogenesis and correlates with late stages of the disease［J］.Int J Cancer,2011,128(2):266-273.
［8］ Hynninen P,Parkkila S,Huhtala H,et al.Carbonic anhydrase isozymes Ⅱ,Ⅸ and Ⅻ in uterine tumors ［J］.APMIS,2012,120(2):117-129.
［9］ 王晨滨,李富,李美荣.肺癌肿瘤抑制因子-1在肿瘤中的研究进展［J］.包头医学院学报,2013,29(1):116-119.
［10］ He G，Lei W，Wang S,et al.Overexpression of tumor suppressor TSLC1 by a surviving-regulated oncolytic adenovirus significantly inhibits hepatocellular carcinoma growth ［J］.Cancer Res Clin Oncol,2012,138(4):657-670.
［11］ 林称意,原野,罗卫民,等.TSLC1和CD105 在食管癌组织中表达的临床意义［J］.实用癌症杂志,2014,29(3):263-271.

Memo

Memo:
河南省科技攻关计划项目（编号：142102310464）；漯河医学高等专科学校自然科学项目（编号：2013，Y-J You）
Last Update: 2015-02-02