|Table of Contents|

Effects of 6MV X-ray on apoptosis and cell cycle progression of human hepatoma cell line

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2015 06
Page:
752-755
Research Field:
Publishing date:

Info

Title:
Effects of 6MV X-ray on apoptosis and cell cycle progression of human hepatoma cell line
Author(s):
Tao Zhenchao1; Qiu Jun2; Qian Liting1; Wang Mingming3; Wu Aidong1
1.Department of Radiation Oncology，Anhui Provincial Cancer Hospital,Anhui Hefei 230001，China;2.Department of Medical Imaging,Anhui Second Provicial People's Hospital，Anhui Hefei 230022，China；3Department of Nuclear Medicine，Anhui Medical University，Anhui Hefei 230001,China.
Keywords:
X-rays; HepG2 cells; PLC/PRF/5 cells; cell cycle; cell apoptosis
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2015.06.07
Abstract:
Objective:To evaluate effects of radiotherapy on apoprosis and cell cycle in human hepatoma cell line.Methods:Using flow cytometry to determine cell cycle distribution of HepG2 and PLC/PRF/5 cells from 0-24h after exposure to 4Gy 6MV X-ray and at the 24th h after exposure 0-6Gy.Results：24h after exposed of 4Gy 6MV X-ray to HepG2 and PLC /PRF /5 cells,and cell survival rates of HepG2 and PLC/PRF/5 cells were（7.61±0.77）% and（5.63±0.87）%（P＜0.05）,the discrepancy were gradually increased as the time or dosage increased.After HepG2 cells were exposed to 4Gy 6MV X-ray,the percentage of G0/G1 cells decreased to （40.56±1.59）%，and then，it augmented with prolonged time.But G2/M cells were reverse.After PLC/PRF/5 cells were exposed to 4Gy 6MV X-ray,the percentage of G2/M cells augmented to （22.11±1.48）%，and it decreased with prolonged time.The percentage of G2/M cells increased with irradiated dose from 0-6Gy after PLC/PRF/5 cells were exposed to 6MV X-ray and at the 24th h.Conclusion：Irradiation could induce the apoptosis of human hepatoma cell line.The cell cycle progression of HepG2 and PLC/PRF/5 cell lines could be changed by 6MV X-ray with the doses of 2-6Gy.X-irradiation could induce G1 and G2 arrest in HepG2 cells,G2 arrest in PLC/PRF/5 cells.

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Memo

Memo:
安徽省卫生厅医学科研重点项目（编号：2010A004）
Last Update: 2015-02-02