|Table of Contents|

Study on the expression and function of DICER1 in leukemic cell lines

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2014 01
Page:
22-0026
Research Field:
Publishing date:

Info

Title:
Study on the expression and function of DICER1 in leukemic cell lines
Author(s):
Bai Ying; Liu Yanqin; Wang Jigang; Liu Jinghua; Li Shanshan; Zhou Fan
Department of Hematology,General Hospital of Shenyang Military Region,Liaoning Shenyang 110056,China.
Keywords:
leukemia; DICER1; HL-60 cell; KG-1 cell; proliferation; apoptosis
PACS:
R730.21;R733.71
DOI:
10.3969/j.issn.1672-4992.2014.01.07
Abstract:
Objective:To assess the DICER1 expression levels in leukemic cell lines HL-60 and KG-1,and further investigate the effects of silencing DICER1 on cell proliferation and apoptosis in leukemia cell lines.The findings will provide a theoretical basis to reveal the role of DICER1 in the pathogenesis of leukemia.Methods:The mRNA and protein levels of DICER1 in leukemic cell lines HL-60 and KG-1 were detected by real time-PCR and Western blot.Human leukemia HL-60 and KG-1 cells were transfected with an DICER1-shRNA expressing vector by LipofectamineTMLTX.Real-time quantitative PCR and Western blot confirmed DICER1 knockdown in both cell lines.Cell proliferation affected by DICER1 interference was assessed using a standard CCK-8 assay.Cell apoptosis was assayed by flow cytometry.Results:DICER1 expression in leukemia cell lines HL-60 and KG-1 was significantly increased both at the mRNA and protein levels,compared with HEK cells（P＜0.05）.The level of DICER1 mRNA and protein was significantly decreased in both cell lines 5 days following transfection with DICER1-shRNA（P＜0.05）.Cell proliferation experiments revealed that DICER1 silencing inhibited cell proliferation of HL-60 and KG-1 cells（P＜0.05）.Flow cytometry analysis demonstrated that silencing of DICER1 significantly induced apoptosis compared to the controls（P＜0.01）.Conclusions:DICER1 expression level was high in leukemic cell lines HL-60 and KG-1,which may induce leukemia cell proliferation and inhibit apoptosis.

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