|Table of Contents|

Effects of DADS on invasion ability of esophageal cancer cells and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2014 01
Page:
18-0021
Research Field:
Publishing date:

Info

Title:
Effects of DADS on invasion ability of esophageal cancer cells and its mechanism
Author(s):
Yin Xiaoran1; Ma Hongbing1; Zhang Jun1; Wang Xijing1; Feng Cheng1; Liu Kaige2; Dai Zhijun1; Zhang Rong1; Ma Yinan1; Liang Liang1
1.Department of Oncology，the Second Affiliated Hospital of Xi'an Jiaotong University，Shaanxi Xi'an 710004，China；2.The First Affiliated Hospital of Xi'an Medical University,Shaanxi Xi'an 710077,China.
Keywords:
DADS; esophageal cancer; invasion; MMP; TIMP
PACS:
R73-36+1;R735.1
DOI:
10.3969/j.issn.1672-4992.2014.01.06
Abstract:
Objective:To investigate the effect and mechanism of diallyl disulfide(DADS) on invasion ability of esophageal cancer cells in vitro.Methods:MTT assay was executed to determine the inhibitory effect of DADS on ECA-109 cells,transwell chamber assay was used to determine DADS-induced effect on invasion ability of esophageal cancer cells.Western blot was used to detect DADS-induced effect on expression of MMP-2,MMP-9,TIMP-1,TIMP-2 protein in esophageal cancer cells.Results:DADS illustrate an inhibitory effect on ECA-109 cells over the concentration of 30μg/ml,DADS could significantly reduce the number of esophageal cancer cells through the invasion chamber in a dose-dependent manner.The expression levels of MMP-2,MMP-9 in esophageal cancer cell were reduced and at the same time the expression of TIMP-1,TIMP- 2 levels were significantly enhanced by DADS.Conclusion:DADS possess an ability of reducing invasion capacity of esophageal cancer cells,inhibiting the expression levels of MMP-2,MMP-9,and enhancing the levels of TIMP-1,TIMP-2.

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Memo

Memo:
陕西省社发攻关项目（编号：2010k01-141）
Last Update: 2013-12-03